Programme Séminaire

LABWORKS LIMS ES: make your data more valuable
Peter Asmus; PerkinElmer (Schweiz) AG

The new LABWORKS Enhanced Security (ES) version gives current users in the environmental, petrochemical, government and other industries the ability to develop more efficient paperless business processes and brings LABWORKS into 21 CFR Part 11 compliance. The new version builds on the existing strengths of LABWORKS, such as fast implementation, low cost of ownership and little need for programmatic customization, and by introducing a centralized point of authentication LABWORKS ES dramatically simplifies the process of securing critical laboratory data.
The significant LABWORKS advantage is a three-tier architecture with database portability that uses the same code regardless of the database or operating system it is being run under. This feature makes it easy, for example, to start out with an inexpensive Access database and migrate to Oracle, as one’s needs increase while avoiding the need for re-coding or retraining users. LABWORKS ES continues a 15- year record of providing tools that make it possible to easily and inexpensively migrate configurations and data from one version to the next.

GEMFID : la nouvelle génération de générateur pour FID
Huu-Phuoc Nguyen, BON Technologies SA

FID a toujours été le détecteur le plus populaire. Il utilise une flamme Hydrogène-air, provenant de sources séparées. Pourtant, l’électrolyse de l’eau génère un mélange 2 :1 v/v d’Hydrogène-Oxygène, qui peut directement alimenter la flamme du FID, sans séparation. C’est le mode de fonctionnement du générateur GEMFID.
La flamme de prémélange H2-O2 offre des avantages en économie de gaz et en sensibilité de détection. Des résultats d’analyse avec la nouvelle version GEMFID sont présentés.
The new Chromatography Systems from Thermo: An overview of LC, LC/MS et GC, GC/MS Systems
Dr. Robert Stoop, Brechbühler AG

Thermo is one of the most innovative companies in the field of analytical instrumentation. This year's news will be presented in more detail: The TRACE GC ULTRA is the new platform for faster and more sensitive analysis. The Surveyor MSQ is the smallest benchtop single Quadrupole LC/MS System with powerful performance. The Trace DSQ, already introduced in 2002 has become a success and one of the most wanted GC/MS Systems available today; some news will be presented here, too.

Instrument Validation and Preventive Maintanance Spare Parts
Ruedi Schwarz, sertec electronics

Identification et quantification par GC-MS de PAH "lourds" dans des matrices complexes
Jean Jacques Sauvain, IST Lausanne

Les émissions diesel et plus particulièrement les petites particules (suies) posent un problème de santé, car elles sont considérées comme probablement cancérigène pour l’homme. La composition des suies diesel est très complexe et plusieurs centaines de produits y ont été identifiés. Parmi ceux-ci, des hydrocarbures aromatique polycycliques (HAP) cancérigènes représentent une grande proportion.
La présentation illustrera les difficultés pour l’analyse de tels composés dans cette matrice par GC-MS en se focalisant plus particulièrement sur des HAP de haut poids moléculaire. L’identification et la quantification de tels HAP sur la base de 4 colonnes différentes (DB-5, BPX-35, BPX-50 et Rtx-200) sera également présentée.
Micro HPLC
Dr. Jürgen Maier-Rosenkranz, GROM GmbH

Only a small number of the common HPLC systems can be used for Microbore HPLC. At flow rates of < 50 µL / min the necessary performance can not be received with these systems . The increasing number of LC/MS applications in the analytical labs provided that more and more systems for Micro LC are available.
The first part gives a description of the different techniques for performing low flow rates and their advantages and weaknesses of this techniques. Especially for the users of such systems procedures will be described for their evaluation.
In the second part an overview about the common available systems will be given.

Capillary-LC - a new Dimension in HPLC
Bernd Pfeffer, SGE (Deutschland) GmbH

The term Capillary-LC is used for HPLC columns with an ID of less than 500 µm. We will be looking at aspects of using these columns in LC/MS systems, especially the higher sensitivity and the small need of solvent.

Workshop GC

ULTRAFAST GC + GC/MS: GC Analysis in Minutes or Seconds (Concept of FAST GC, Technical Requirements, Examples)
Dr. Robert Stoop, Brechbühler AG

GC is an established technique which is widely spread out in all the labs. Yet not much has been said about the GC Techniques recently although there is still enough development going on worth to be mentioned.
One of these newer techniques is the ULTRAFAST GC which allows to cut GC analysis times down to seconds or a few minutes! ULTRAFAST is not just simple FAST GC. In this workshop the different techniques conventional, fast and ULTRAFAST GC will be compared with each other. The possibilities of the ULTRAFAST GC method and it’s application in practice will be further discussed in this workshop.

Gas Generators for the Use in Gas Chromatography
Joyce Link, Milian SA

Gas generators have become a widely accepted substitute for expensive and dangerous gas cylinders in the laboratory. Not only has the safety aspect led people to decide on using the gas generator; it’s especially the high quality with which gas is generated – ultra pure and almost absolutely dry. Be it as feed gas for the FID, ensuring lowest possible background noise, or as carrier gas - it definitely enhances reproducability of your detections.
With the aid of the Parker Balston Gas Generator, we will show you how compact, reliable and easy to maintain today's gas generators can be, and that they fully comply with the laboratory quality standards and regulations.

MassLib exploite mieux les analyses
Peter Kofel, MSP Kofel

La spectrométrie de masse, aussi bien qu’elle est établie comme méthode pour la détermination du poids moléculaire, est rarement appliquée pour l’élucidation de la structure chimique. La raison est bien simple : trop fastidieux et difficile paraît le processus de l’interprétation du spectre au non-spécialiste et plutôt non-utilisable pour ce processus se présentent les logiciels basés sur des recherches de spectres en bibliothèques.
MassLib en est une exception : ses recherches en similarité offrent de reconnaître des aspects structuraux même si les spectres nous paraissent fortement dissimilaires. Des recherches de fragments, neutres et structures en combinaison permettent d’attaquer le problème de structure inconnue aussi là où aucune quantité suffisante d’échantillon permettrait d’autres méthodes que la spectrométrie de masse.
Pour l’exploitation des analyses de spectrométrie de masse avec MassLib non seulement des traces internes (par exemple des traces single ion) et externes (par exemple des traces de température) peuvent être utilisées, mais aussi des analyses de comparaison. La comparaison d’analyses entières assistée par le logiciel permet de reconnaître et d’interpréter des différences rapidement même si deux analyses sont très similaires.

Workshop HPLC

Validation of Detectors made easy
Nicole Stutz, Celectole

Thanks to their good training, lab technicians know how to replace spare parts in HPLC- and GC Systems and how to check that the systems keeps running conform to regulations.
eg. exchanging a deuterium lamp in an HPLC Detector and testing the correct UV wavelenght.
It is important to avoid mistakes and to document each step taken. We will introduce a software which provides HPLC and GC users with an excellent tool.

Examples for the HPLC user are testing:+ correct flowrate
+ correct gradient
+ correct UV-wavelength
+ linear UV-adsorbtion
+ correct temperature of the column oven
+ correct functioning of the autosampler

With the test for a correct flowrate, we will explain the software and the test report in detail.
You can choose a test to test the advantages of the software yourself.

Chiral Method Development
Dr. Annette Dibowski, YMC GmbH, Dr. Harald Dibowski, YMC GmbH

The separation of enantiomeric substances is challenging, because these compounds have similar characteristics in a non-chiral enviroment. Therefore melting points, chemical and spectroscopic characteristics are identical. As a result these enantiomers can not be separated under standard normal phase or reversed phase chromatographical conditions. In a chiral enviroment these chiral substances behave differently, which can be used for the separation of enantiomeric compounds.
For a better understanding of the different separation mechanisms with so called Chiral Stationary Phases these selectivities can be divided into types according to their different interaction mechanisms and supports (e.g. silica, cellulose etc.).
All individual types will be discussed and examples for CSPs and corresponding applications will be shown. The most important conditions for successful chiral separations will be presented.