Seminario

Sugar Analysis of food with a polymeric column
Thomas Orthmann, Showa Denko Europe GmbH

HPLC applications of sugar in food using an Amino column will be introduced. The packing material is polymer. The surface is modified with amino functional group (polymer-based NH2).
Usually, silica-based NH2 columns are used for the HPLC analysis of sugars when reversed phase mode is applied. However the life time of the silica-based NH2 column is not very long. The short life time is due to damage to the base material of silica gel by its NH2 group. Silica material is easy to be dissolved by the alkaline solution. The functional group, -NH2, is a type of alkaline modifier and it leads inside of the column to the alkaline atmosphere. This decreases the column performance.
Using polymer-based packing material, we could keep the inside column atmosphere stable, therefore, the life time is longer. Additionally, this polymer based column also shows high sensitivity.
The application data for the analysis of sugars in foods using tough NH2 gel, Shodex Asahipak NH2P-50, is to be shown. Furthermore, real sample applications, especially the sample pre-treatment and useful advices for sugar analysis is going to be demonstrated.

Soluzioni Strumentali per il campionamento e l'analisi dell'aria
Manuela Bergna, Dani Instruments

There is a still growing interest around the world in monitoring Volatile Organic Compounds in air.
The awareness of their effect on human health brought new regulations and better air –monitoring methods. The demand for instrumentation capable to fulfil these analytical requirements is also increasing.
This lecture shows analytical configurations based on thermal desorption and capillary gas chromatography that cover the wide range of applications. Instrumental solutions are presented that perform both off-line and on-line monitoring of VOCs.

Cleaning Validation: Validazione e certificazione di processi di lavaggio di precisione peri prodotti della meccanica fine mediante analisi GC e GC-MS dei residui oleosi a livello di tracce.
Carlo Devittori, Liceo Lugano 2, Savosa/Scuola Universitaria Professionale della Svizzera Italiana, Dip. Tecn. Innovative, Manno

Nel lavoro qui descritto è stato sviluppato in via preliminare un metodo analitico che permetta di quantificare residui di oli lubrificanti su superfici destinate all’implantologia medica e alla meccanica di precisione, per le quali è richiesto un grado di pulizia definito come “precision clean” (< 10 µg/cm2). Questo metodo analitico, basato sull’adsorbimento dei componenti volatili e semivolatili dei residui oleosi su microfibre e sulla loro analisi successiva mediante gascromatografia e spettrometria di massa, ci ha permesso di misurare in modo riproducibile residui superficiali di olio dell’ordine di 1 µg/cm2. Questo processo può essere implementato a livello industriale per la validazione di metodi di lavaggio e per la certificazione dei lotti prodotti. Rispetto ai metodi tutt’ora impiegati e a quelli (anche molto sofisticati) descritti in letteratura esso permette di ottenere una risposta quantitativa assoluta riferibile anche alle superfici non direttamente esposte, che rappresentano l’ambito maggiormente problematico nei processi di lavaggio: infatti, pongono problemi di accessibilità a solventi e a detergenti e problemi di evacuazione degli oli minerali relativamente viscosi; inoltre, queste superfici non sono analizzabili in modo attendibile con metodi ottici o con metodi indiretti.

Piu sensibilità – Migliore separazione – Tempi d’analisi minimali? - Ce cosa sì po’ aspettare delle tecniche CG e CG/SM oggi?
Dr. Robert Stoop, Brechbühler AG

There is still a lot of development and new techniques in the field of GC and GC/MS. An overview of the latest developments and improvements will cover all important elements from the injectors to the columns and last but not least to the detectors (mainly MS)
Large volume injection (LVI) is now available for all injectors (Cold on Column, PTV, and Splitless). LVI Injection is a very powerful technique to reduce sample preparation time and to better integrate the whole solvent evaporation step into the chromatography which is far more accurate than manual evaporation steps. On the other hand, LVI can improve sensitivity drastically by a factor of 100 xs or more.
The Impact of Fast and Ultra-Fast Chromatography on productivity and on analysis time will be shown with real live applications in the environmental field (H53 method) and food and flavour analysis.
A well known technique, GCxGC is now commercially available and the advantage of this powerful technique will be presented. Apart from increasing the peak capacity by a factor of 10 or more it also offers a much more descriptive picture when using complex mixtures as peaks are typically ordered by boiling point and by polarity.

TurboMatrix HS Trap - Redefining Detection Limits
Dr. Peter Hoedl, PerkinElmer (Schweiz) AG

Workshop GC

GCxGC Data Treatment: The possibilities of GCxGC will be shown with a current example. The requirements for data treatment with GCxGC are much bigger than with one-dimensional GC. This workshop will show why.
Dr. Robert Stoop, Brechbühler AG

GCxGC Data Treatment: The possibilities of GCxGC will be shown with some concrete examples.
The requirements for GCxGC data treatment are much higher than those of conventional GC. This workshop will show, why. Examples of GCxGC/FID and also GCxGC/MS will be shown

Rapid and Accurate Ion Analysis in Cola Drinks
Urs Waldburger, Metrohm AG

A method is presented for the rapid and extremely accurate determination of phosphoric acid in cola drinks using ion chromatography (IC). As a result of the specially optimized application and instrument configuration even high concentrations can be measured directly and exactly; the method can therefore be used for quality control.
In addition, IC offers a wide range of applications for numerous further uses in the analysis of drinks. In comparison to the classical methods, e.g. photometry or ISE, several parameters can be determined simultaneously. With suitable preparation techniques (e.g. inline dialysis or ultrafiltration) it is possible to dispense with expensive and time-consuming sample preparation steps.

Changing septa, liner and capillaries: a practical training on a PerkinElmer GC
Peter Pichler, AccyChrom/infochroma ag

Vials and Caps
- how to chose the best suited vial (Volume, Shape, Dead Volume)
- discussion of closure options (Crimp, Snap/Crimp or Screw Cap)
- Septa and their usage
Injection Techniques
- GC Autosampler
- LC Autosampler
- manual injection

Chosing the right Syringe
- Volume, Plunger, Needle
- Autosamler Syringes

Septa for GC Injectors
- material and the influence of temperature

Liners für GC Injectors
- inner diameter
- plugs of glass/-quartz wool
- Focus-Liner
GC Capillary Connections
- cutting the column
- Ferruls and their usage

Chosing a capillary column
Endcapping of LC Columns
- introducing Opti-Lynx

Workshop HPLC

Dilution and Dispensing Techniques for Sample preperation for HPLC and GC Applications
Joyce Link, Milian SA

Sample Preparation for LC/MS Analysis: Using Capillary Trap Cartridges to desalinate and re-buffer samples
Ruedi Schwarz, sertec electronics

To desalinate or re-buffer samples is a re-ocurring problem in LC/MS analysis. There are many ways to prepare a sample. But often these procedures are time consuming or cause sample loss.
Using Trap Cartridges is often the best way to excellent analysis results and a higher sample throughput.
Two Trap Cartridges will be introduced:
OPTI-PAK capillary trap cartridges for very small volume applications
bedvolume: 0.12 µl to 5.0 µl
OPTI-LYNX trap cartridges for higher capacities. They go with the patented Quick-Connect hardware which allows the fast and secure changing of the trap cartridge.
bedvolume: 4 µl to 100 µl